Journal: Journal of Biological Engineering

Article Title: Designing of PLA scaffolds for bone tissue replacement fabricated by ordinary commercial 3D printer

doi: 10.1186/s13036-017-0074-3

Figure Lengend Snippet: Confocal microscopy photomicrographs of ST1 and ST2 seeded with osteosarcoma cells. Confocal microscopy photomicrographs of ST1 ( a , c ) and ST2 ( b , d ) scaffolds from polylactic acid seeded with osteosarcoma cells MG-63 after a 7-day and 14-day culture. Immunohistochemical staining using monoclonal antibody against either type I collagen ( a , b ) or osteocalcin ( c , d ), followed by secondary antibody conjugated with Alexa Fluor 488® ( green ) and propidium iodide staining of cell nuclei ( red ) showed groups of cells producing type I collagen on both scaffolds ( a , b ) after 7 days, but only rare osteocalcin staining in both scaffolds ( c , d ) after 14 days. Objective ×10×, magnification ×4, bar = 20 μm

Article Snippet: Immunohistochemical staining was performed using mouse monoclonal antibody against osteocalcin (overnight, 2–8 °C, dilution 1:200, ab13420, Abcam) or mouse monoclonal antibody against type I collagen (concentrate, overnight, 2–8 °C, dilution 1:20, clone M-38c was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242) and subseaquently with secondary anti-mouse antibody conjugated with AlexaFluor® 488 (45 min RT, dilution 1:300, A10667, Life Technologies).

Techniques: Confocal Microscopy, Immunohistochemical staining, Staining

Journal: Materials Today Bio

Article Title: The combination of a 3D-Printed porous Ti–6Al–4V alloy scaffold and stem cell sheet technology for the construction of biomimetic engineered bone at an ectopic site

doi: 10.1016/j.mtbio.2022.100433

Figure Lengend Snippet: Gene primer sequences for RT‒PCR of BMSCs.

Article Snippet: Primary antibodies, including anti -OCN (Genetex, 1:1000), anti -OPN (Novusbio, 1:1000), anti-COL-I (Bioss, 1:1000), anti -Runx2 (Bioss, 1:1000), and anti -β-actin (Abcam, 1:1000), were added to the membranes.

Techniques:

Journal: Materials Today Bio

Article Title: The combination of a 3D-Printed porous Ti–6Al–4V alloy scaffold and stem cell sheet technology for the construction of biomimetic engineered bone at an ectopic site

doi: 10.1016/j.mtbio.2022.100433

Figure Lengend Snippet: In vitro analysis of osteogenesis of the combination of BMSCs and PT scaffolds. (A) Schematic of the experiment. BMSCs were collected from each group after 7 days and 14 days of coculturing with PT scaffolds in osteogenic induction medium. (B) Alizarin red staining images. (C) Quantification of Alizarin red staining based on the relative absorbance value. (D–G) The RT‒PCR results of osteogenesis-related gene expression of OCN, OPN, COL-I, and Runx2 in the PT, PT/LC, PT/MC, PT/HC, and PT/CS groups on days 7 and 14. These results suggested that increasing the number of initial seeding cells improved the capability of osteogenic differentiation. (H) Western blot (WB) analysis presented results similar to those of RT‒PCR. ∗ indicates significant differences (p ​< ​0.05).

Article Snippet: Primary antibodies, including anti -OCN (Genetex, 1:1000), anti -OPN (Novusbio, 1:1000), anti-COL-I (Bioss, 1:1000), anti -Runx2 (Bioss, 1:1000), and anti -β-actin (Abcam, 1:1000), were added to the membranes.

Techniques: In Vitro, Staining, Gene Expression, Western Blot